Isolation and detailed characterisation of the first sterigmatocystin hyperproducer mould strain in Hungary

Abstract Aspergillus strains were isolated from Hungarian mills in order to get information on the appearance of sterigmatocystin (ST) producing moulds, whose presence has never been demonstrated Hungary. Fungal isolates classified into nine morphotypes, sections Nigri , Nidulantes Versicolores (two morphotypes), Circumdati Flavi Clavati and Terrei by classical mycological assays. ST could be section . production was assessed liquid solid phase growth experiments compared reference strains: pepii SzMC 22332, versicolor 22333, griseoaurantiacus 22334 nidulans RDIT9.32. Four our marked as Km11, Km14, Km26 Km31 showed medium. corn grit substrate measured after three weeks incubation, isolate proved most prominent with a toxin concentration 277.1 ?g g ?1 surpassing all strains. The toxin-producing ability also tested field experiment, where infected. By end level 19.56 kg infected corn. Molecular taxonomic identification strain performed using internal transcribed spacer (ITS), calmodulin tubulin sequence analyses. Based these studies, identified creber Here we report that an ST-producing A. appeared Hungary, is first known extreme mould this country. As result climate change, aflatoxin B1 flavus have Hungary last decade. only so far, there no sign mass prevalence, due lower temperature optimum species its probably not related change.